کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1227947 | 968440 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Application of non-aggressive sample preparation and electrothermal atomic absorption spectrometry to quantify platinum in biological matrices after cisplatin nanoparticle administration Application of non-aggressive sample preparation and electrothermal atomic absorption spectrometry to quantify platinum in biological matrices after cisplatin nanoparticle administration](/preview/png/1227947.png)
A reproducible, simple, rapid and sensitive electrothermal atomic absorption spectrometry (ETAAS) method using low volume of sample (< 50 μL) was described for the quantitative analysis of cis-diamminedichloroplatinum (II) (cisplatin) in several biological matrices after PLGA [Poly (d,l-lactic-co-glycolic acid)] cisplatin nanoparticles (NP) administration to tumor-bearing mice. The validation parameters such as specificity, linearity, accuracy and precision obtained in this method were according to FDA guidelines and are presented in the text, as well as the limit of detection and quantification. Two applications were carried out with this method. In-vitro application was used to measure the loading efficacy of cisplatin in the formulation, and to characterize the drug kinetic release in culture cell medium. For in-vivo application, three groups of nu/nu mice injected with tumor cells were treated with 5 mg kg− 1 dose of free cisplatin by intravenous and intraperitoneal routes, respectively, and with NP by intraperitoneal route. Blood samples were collected at different times, to measure cisplatin plasma concentrations, and at the end of the study, different organs were removed from each animal to quantify drug distribution. Additionally, the relationship between cisplatin levels and its apoptotic activity in each tumor sample, could be also characterized. This simple and sensitive method without aggressive manipulation allowed the quantification of cisplatin in different biological matrices with independence of the formulation used. Therefore, these pre-clinical results show the possibility to include this method for clinical applications.
Journal: Microchemical Journal - Volume 96, Issue 2, November 2010, Pages 415–421