کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1228467 | 968469 | 2007 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Towards the characterization of metal binding proteins in metal enriched yeast
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
This paper presents size exclusion chromatography data with on-line coupling to UV and inductively coupled plasma mass spectrometry (ICP-MS) of water soluble metal-binding compounds present in zinc, copper, chromium and iodine enriched yeast nutritional supplements. Molecular weight estimates of the extracted metal-containing compounds are given and are shown to vary substantially from 1.2 kDa to larger than 668 kDa. Seven proteins suspected of containing chromium were identified from one of the chromium-containing fractions. Four of these identified proteins are known to form complexes with other metal ions. The metal chromatographic profiles of zinc, copper and chromium-enriched yeasts were compared to their respective native metal profiles in non-enriched yeast samples. The chromium profiles are shown to be markedly different while those of zinc and copper are qualitatively similar. Only iodide ions or weakly bound, non-aromatic, low molecular weight (â¼Â 1.2 kDa) iodine species were observed in the iodine-enriched yeast samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 85, Issue 2, April 2007, Pages 329-340
Journal: Microchemical Journal - Volume 85, Issue 2, April 2007, Pages 329-340
نویسندگان
Tamer Shoeib, Zoltán Mester,