کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1250690 1496046 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of atorvastatin in human serum by salting out assisted solvent extraction and reversed-phase high-performance liquid chromatography–UV detection
ترجمه فارسی عنوان
تعیین آتورواستاتین در سرم انسان با استفاده از استخراج حلال کمکی و کروماتوگرافی مایع با کارایی بالا
کلمات کلیدی
آتورواستاتین، خالص سازی از استخراج حلال کمک می کند، پاک کردن، پیش انقباض، سرم
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی

A simple and rapid technique based on salting out assisted solvent extraction was developed for extraction of atorvastatin from serum sample and high performance liquid chromatography–UV was used for its detection. In the present study, 1.0 mL serum was extracted by 0.5 mL of acetonitrile and some parameters that can affect extraction such as type and volume of extraction solvent, type of salt, and pH were optimized. Under optimized experimental conditions, the calibration curve was found to be linear in the range of 0.001–10 ng mL−1 in human serum and the correlation coefficient (R2) and the limits of detection were >0.99 and 0.0005 ng mL−1, respectively. The accuracy of the method in terms of average recovery of the compound in spiked serum and water samples was better than 90%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Arabian Journal of Chemistry - Volume 7, Issue 1, January 2014, Pages 87–90
نویسندگان
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