کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1251092 | 970885 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Method
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
According to the amino acid sequence and codon preference of E. coli, the human interleukin-18(IL-18) gene was optimized to avoid the rare codons. The total length of the synthesized gene is 571 bp; 18 oligonucleotides, DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method. The whole DNA sequence was synthesized by a one-step total gene synthesis method, and then inserted in pUC18 vector. Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co., Ltd. for sequencing. The sequencing result shows that one clone contained the complete correct gene in all the five positive clones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Research in Chinese Universities - Volume 24, Issue 4, July 2008, Pages 487-490
Journal: Chemical Research in Chinese Universities - Volume 24, Issue 4, July 2008, Pages 487-490
نویسندگان
Chao-hui GAO, Xiao-yue SHI, Xin-tong HOU, Qing-fan MENG, Ying-jiu Zhang, Li-rong TENG,