کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1251190 1496053 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of efficient SPE–TLC method and evaluation of biological interactions of contraceptives with progesterone receptors
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Development of efficient SPE–TLC method and evaluation of biological interactions of contraceptives with progesterone receptors
چکیده انگلیسی

TLC–SPE methodologies were developed to ascertain biological interactions of norethindrone acetate and dydrogesterone contraceptives with plasma progesterone receptor proteins. TLC solvent system for plain and Cu(II) impregnated silica gel plates was n-hexane-n-butanol (90:10, v/v), which took 20 min to run up to 10.0 cm. The best separation was on Cu(II) impregnated plates due to maximum difference in Rf values and compact spots. The optimized SPE conditions were pH 2.0 and 3.0 of phosphate buffer (50 mM) for norethindrone acetate and dydrogesterone, respectively. The flow rate of plasma and eluting solvent (methanol) through C18 cartridge was 0.10 mL/min. The interactions of these contraceptives with progesterone receptor proteins were analysed by TLC–SPE results, which were supported by modelling using PyMOL and Autodoc4 softwares. The dydrogesterone was found to be bound strongly than norethindrone acetate. Attempts have been made to discuss the drugs’ interactions at chemo-supramolecular level.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Arabian Journal of Chemistry - Volume 5, Issue 2, April 2012, Pages 235–240
نویسندگان
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