کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1251443 | 1496067 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chemoenzymatic Synthesis of Cellular Adhesion Tripeptide RGD Precursor in Organic Media1
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chemoenzymatic synthesis of tripeptide Bz-RGD-(OEt)2 was conducted in this study. First, the free dipeptide Gly-Asp was synthesized via a novel chemical method, wherein only L-aspartic acid was used and was followed by the esterification of Gly-Asp. The formation of the linkage between the third amino acid Bz-Arg-OEt and Gly-Asp-(OEt)2 was completed by using the enzymatic method in organic media. The effects of several factors such as pH, the water content, triethylamine(TEA), the molar ratio of the substrates, and the reaction time on the yield of Bz-RGD-(OEt)2 were examined. It was obtained that the optimum conditions for Bz-RGD-(OEt)2 synthesis in an ethanol/ Tris-HC1 buffer system (volume ratio 93: 7) were as follows; pH=8.0; temperature, 30 °C; reaction time, 7 h. The tripeptide yield was 75. 2%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Research in Chinese Universities - Volume 22, Issue 6, November 2006, Pages 721-726
Journal: Chemical Research in Chinese Universities - Volume 22, Issue 6, November 2006, Pages 721-726
نویسندگان
WANG Hua, ZHAO Mi-feng, PU Li-mei, DING Wei, HUANG Yi-bing, HOU Rui-zhen, ZENG Hong-bin, XU Li, ZHANG Xue-zhong,