کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1252893 | 1496057 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Sensitive liquid chromatography–tandem mass spectrometry method for the determination of pantoprazole sodium in human urine Sensitive liquid chromatography–tandem mass spectrometry method for the determination of pantoprazole sodium in human urine](/preview/png/1252893.png)
A sensitive and selective liquid chromatographic–tandem mass spectrometric (LC–MS–MS) method was developed to determine pantoprazole sodium (PNT) in human urine. After solid-phase extraction with SPE cartridge, the urine sample was analysed on a C18 column (symmetry 3.5 μm; 75 mm × 4.6 mm i.d) interfaced with a triple quadrupole tandem mass spectrometer. Positive electrospray ionization was employed as the ionization source. The mobile phase consisted of acetonitrile–water (90:10, v/v). The method was linear over a concentration range of 1–100 ng mL−1. The lower limit of quantitation was 1 ng mL−1. The intra-day and inter-day relative standard deviation across three validation runs over the entire concentration range was <10.5%. The accuracy determined at three concentrations (8.0, 50.0 and 85.0 ng mL−1 PNT) was within ±1.25% in terms of relative errors.
Journal: Arabian Journal of Chemistry - Volume 4, Issue 2, April 2011, Pages 163–168