کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1262772 | 971895 | 2007 | 5 صفحه PDF | دانلود رایگان |
Nitric oxide (NO) and its reaction products were key players in the pathophysiology of sepsis and shock. The present study was designed to explore the effects of lanthanum chloride (LaCl3) on inducible nitric oxide synthase (iNOS) expression, at both gene and protein levels, in RAW264.7 macrophages induced by Lipopolysaccharide (LPS). Reverse transcription polymerase chain reaction (RT-PCR), immunofluorescence, and western blot were employed to measure iNOS gene expression, localization, and protein expression respectively. NO production in culture supernatants was detected by the nitrate reductase method. The results showed that LaCl3 significantly attenuated the iNOS gene and protein expression, as well as NO production in RAW264.7cells induced by LPS.
Journal: Journal of Rare Earths - Volume 25, Issue 3, June 2007, Pages 359-363