کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1266092 972261 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An efficient enzymatic modification of cordycepin in ionic liquids under ultrasonic irradiation
ترجمه فارسی عنوان
اصلاح آنزیمی کوردیپسین در مایعات یونی تحت اشعه ماوراء بنفش
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
چکیده انگلیسی


• Enzymatic acylation of cordycepin in ILs under ultrasound was investigated.
• Appropriate ultrasound power can accelerate enzymatic reaction.
• Much better enzyme performance was acquired under ultrasonication than shaking.
• Biocatalyst displayed excellent regioselectivity and high stability in IL.

A comparative study of the immobilized Candida antarctica lipase B (Novozym 435)-catalyzed acylation of cordycepin with vinyl acetate in ionic liquids (ILs) under ultrasonic irradiation and shaking was conducted. The application of ultrasonic irradiation instead of shaking during acylation resulted in an enhanced reaction rate and a higher level of substrate conversion. Among the various ILs examined, 1-butyl-3-methylimidazolium tetrafluorobrate ([C4MIm][BF4]) was the best medium for the reaction because it produced the highest substrate conversion. In [C4MIm][BF4], the optimal ultrasonic power, water activity, and reaction temperature were 120 W, 0.33, and 50 °C, respectively. The acylation of cordycepin in [C4MIm][BF4] proved to be regioselective under both conditions: the C5′-OH was acylated. Novozym 435 exhibited a much higher operational stability in [C4MIm][BF4], and 58.0% of its original activity was maintained after ten reuse cycles under ultrasonic irradiation. Compared with the cordycepin, the rate of adenosine deaminase-catalyzed deamination was greatly reduced when the 5′-OH was substituted by acetyl group. These results demonstrated that the combined application of ultrasonic irradiation and IL as a medium was an efficient approach for the enzymatic modification of cordycepin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasonics Sonochemistry - Volume 21, Issue 5, September 2014, Pages 1682–1687
نویسندگان
, , , , ,