The influence of ultrasound on the fluoroquinolones antibacterial activity

In this work, the antibacterial effect of fluoroquinolones (FQs) upon Escherichia coli (E.coli) was measured with and without application of 40 kHz ultrasound (US) stimulation. The research results demonstrated that simultaneous application of 40 kHz US apparently enhanced the antibacterial effectiveness of FQs. That is, the synergistic effect was observed and the bacterial viability was reduced when FQs and US were combined. In addition, various influencing factors, such as FQs drug concentration, US irradiation time and solution temperature, on the inhibition of E.coli were also investigated. The antibacterial activity was enhanced apparently with increasing of FQs drug concentration, US irradiation time and solution temperature. Furthermore, we discussed preliminarily the mechanism of US enhanced antibacterial activity. Results show that US can activate FQs to produce reactive oxygen species (ROS) indeed, which are mainly determined as superoxide radical anion (·O2−) and hydroxyl radical (·OH).

Research highlights
► The 40 kHz US apparently enhanced the antibacterial effectiveness of FQs.
► Various influencing factors on the inhibition of E.coli were investigated.
► We discussed preliminarily the mechanism of US enhanced antibacterial activity.
► Results show that US can activate FQs to produce ROS indeed.