کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1313990 | 1499335 | 2014 | 4 صفحه PDF | دانلود رایگان |
• The elution order of the analytes corresponds to their eluent-philicity.
• Optimal separation was achieved through hetero-pairing of column and eluent.
• Separation is further improved by elevated temperature.
• Separation in fluorous mixture synthesis is based on the fluorine content percentage F% rather than the number of fluorine atoms.
Using the set of fluorinated amphiles that contain the same fluorocarbon moiety but differ in their fluorine content percentage F% (25–45%), the optimal condition for a F%-based separation of these analytes using reverse-phase chromatography was explored. It is found that optimal separation can be achieved by pairing a regular reverse-phase column (such as C8) with a fluorinated eluent (such as trifluoroethanol). Separation is further improved at higher chromatographic temperature with baseline separation achieved at 45 °C. This result indicates that the separation of fluorocarbon-tagged molecules can be based on the fluorine content percentage rather than the number of fluorine atoms.
Optimal separation of fluorinated amphiles with HPLC can be achieved by pairing a regular reverse-phase column with a fluorinated eluent and separation is further improved by elevated temperature.Figure optionsDownload as PowerPoint slide
Journal: Journal of Fluorine Chemistry - Volume 165, September 2014, Pages 39–42