کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1315175 976008 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and surface properties of new semi-fluorinated sulfobetaines potentially usable for 2D-electrophoresis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Synthesis and surface properties of new semi-fluorinated sulfobetaines potentially usable for 2D-electrophoresis
چکیده انگلیسی

New semi-fluorinated amidosulfobetaines, homologs of hydrocarbon amidosulfobetaines (ASB) commonly used in two-dimensional gel electrophoresis (2DE), were prepared in three steps from 2-F-alkylethyl iodide or F-alkyl iodide. Their synthesis was described and their air–water interface properties were investigated and compared with their perhydrogenated counterpart properties. The influence of the relative lengths of the perfluorinated and hydocarbonated moieties was discussed. 2DE of a rat testicular membrane fraction was performed comparatively using one of these fluorinated sulfobetaines and its hydrocarbon homolog; these preliminary results showed the great potential of the semi-fluorinated sulfobetaines in proteomic analysis.

Semi-fluorinated counterparts (FASB-p,m) of amidosulfobetaines ASB-n were synthesized, their surface properties compared and their great potentiality in proteomic analysis demonstrated using 2D electrophoresis. Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Fluorine Chemistry - Volume 128, Issue 3, March 2007, Pages 211–218
نویسندگان
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