Asymmetric reduction of ketones by Geotrichum candidum: immobilization and application to reactions using supercritical carbon dioxide

The enantioselectivity for the reduction of ketones by Geotrichum candidum NBRC 5767 was improved upon immobilization of the whole cell onto an ion exchange resin with polyallylamine. Furthermore, immobilization of the cell enhanced the stability of the enzyme and enabled a continuous-flow reaction under normal aqueous conditions. The biocatalyst was also applied to the reaction in supercritical carbon dioxide.

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