کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1348181 | 980343 | 2008 | 11 صفحه PDF | دانلود رایگان |
During the synthesis of new caspase substrates, we have encountered extensive aspartate epimerization upon coupling of t-Bu-protected tetrapeptides Ac-IETD-OH or Ac-DMQD-OH with aromatic amines (aminocoumarins and aminoquinolines) by using aminium-based coupling reagent HATU in the presence of 2,4,6-trimethylpyridine (TMP). To study this reaction in more detail, an RP-HPLC method was developed that afforded the separation of the epimers. By carefully adjusting the reaction conditions, the epimerization could be reduced to very small levels (from 75% down to below 3%). A new, highly hindered base 2,4,6-tri-tert-butyl-pyridine (TBP), was found to be superior to the traditional TMP in the catalysis of this reaction. Moreover, the aspartate epimers were found to be interconvertable at elevated temperatures in an inert solvent, whereby the d-aspartate containing epimer was the thermodynamically controlled product. Over the course of the study, new side products of HATU coupling reactions, the N,N-dimethylamides of the aspartic residue of above tetrapeptides, were also identified.
Figure optionsDownload as PowerPoint slide
Journal: Tetrahedron: Asymmetry - Volume 19, Issue 1, 30 January 2008, Pages 49–59