Enantioselective oxidation of thioanisole with an alcohol oxidase/peroxidase bienzymatic system

Optimization of a new bienzymatic couple alcohol oxidase/peroxidase for the asymmetric oxidation of thioanisole was realized. The main advantages in the application of this system is the use of inexpensive and easily available enzymes and substrates, permitting gram scale enantioselective syntheses. Methanol, as a substrate for alcohol oxidase, also facilitates the solubilization of organic substrates in the reaction medium.

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Methyl-phenyl-sulfoxideC7H8OSEe = 75–78% eeSource of chirality: asymmetric enzymatic catalysisAbsolute configuration: (S)