کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1353758 1500400 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of candidate AFLP markers for shell color of the Pacific oyster (Crassostrea gigas) under artificial selection
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Identification of candidate AFLP markers for shell color of the Pacific oyster (Crassostrea gigas) under artificial selection
چکیده انگلیسی


• High level of genetic diversity was observed in four shell color variants of the Pacific oyster.
• There was moderate genetic differentiation among the shell color variants.
• Eleven outliers were detected by the hierarchical analysis and the Bayesian likelihood method in the oysters.

The shell color of the Pacific oyster (Crassostrea gigas) is a desirable trait, but only a few genetic studies on shell color have been documented. Through successive selective breeding, four shell color variants of white (W), gold (G), black (B) and purple (P) C. gigas have been developed. The amplified fragment length polymorphism (AFLP) technique was used to scan the genomes of the four variants with different shell colors and one wild population (C) to identify candidate markers for shell polymorphism. Fifteen AFLP primer combinations were used, 1079 loci were scored as polymorphic loci, and the percentage of polymorphic bands was 95.5%. In the gold, white, black, purple and wild populations, the percentages of polymorphic loci were estimated to be 90.5% (G), 90.0% (W), 91.1% (B), 95.3% (P) and 93.2% (C); the expected heterozygosity values were 0.3115 (G), 0.3044 (W), 0.3102 (B), 0.3285 (P) and 0.3105 (C). The white shell variant was observed to have slightly lower genetic diversity than others, with a FST value of 0.1483. These results indicated that the four different shell color variants had high genetic diversity and that the genetic differentiation of populations mostly results from genetic diversity of individuals within populations. Furthermore, 11 outlier loci were considered candidate markers for shell color. This work provides new insights on relationships among color variants of C. gigas.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Systematics and Ecology - Volume 66, June 2016, Pages 209–215
نویسندگان
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