کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1353775 1500400 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and characterization of 26 polymorphic microsatellite markers in Lateolabrax maculatus and cross-species amplification for the phylogenetically related taxa
ترجمه فارسی عنوان
توسعه و ویژگی 26 نشانگر ریزماهواره پلی مورف در Lateolabrax maculatus و تقویت گونه های متقابل برای تاکسون های مرتبط با فیلوژنتیک
کلمات کلیدی
Lateolabrax maculatus؛ چندینکولیت تکرار شده Polynucleotide؛ تکنولوژی توالی پیشرفته؛ تقویت متقاطع
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی


• 26 new polynucleotide-repeat microsatellite loci were isolated in Lateolabrax maculatus.
• Transferability of 6 loci was positive across 10 phylogenetically related species of Latidae and Serranidae.
• These markers will help to study population genetics and conservation management.

The spotted sea bass Lateolabrax maculatus, a commercially important species of fishery resource in the Northwestern Pacific, has become a popular cultured fish species in China. Although the microsatellite markers of L. maculatus have been isolated, they mainly focused on dinucleotide-repeat loci. In this study, we developed 26 polynucleotide-repeat microsatellite loci for L. maculatus through high-throughput DNA sequencing technology. The number of alleles per locus ranged from 3 to 19. Observed and expected heterozygosities varied from 0.188 to 0.906 and 0.175 to 0.938, respectively. One locus showed significant deviation from Hardy-Weinberg equilibrium after Bonferroni correction. These 26 novel microsatellite markers will serve as useful tools which benefit population genetics studies and conservation management of L. maculatus. In addition, cross-species amplification tests showed that the transferability of 6 loci was positive across 10 species of two phylogenetically related families Latidae and Serranidae, and 3 loci were successful amplified in at least 5 species. The transferable loci would be helpful for the detection of genetic relationship and divergence between the tested taxa.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Systematics and Ecology - Volume 66, June 2016, Pages 326–330
نویسندگان
, , , , ,