کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1392654 1501149 2013 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Modulation of human neutrophils' oxidative burst by flavonoids
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Modulation of human neutrophils' oxidative burst by flavonoids
چکیده انگلیسی


• Flavonoids are effective modulators of neutrophil's oxidative burst.
• Catechol group in B-ring is determinant for this effect.
• Hydroxylations in A-ring of flavonoids are not determinant for this effect.
• A 3′-methoxyl group in B-ring of flavonoids contributes for higher activity.
• The 3-OH in C-ring and the C2–C3 double bound are not mandatory for the activity.

Inflammation is a normal response towards tissue injury, but may become deleterious to the organism if uncontrolled. The overproduction of reactive species during the inflammatory process may cause or magnify the damage at inflammatory sites. Flavonoids have been suggested as therapeutic agents to avoid such damage, as these compounds exhibit anti-inflammatory activity, through the modulation of oxidative stress and signalling pathways. Both effects may attenuate neutrophils' activities at inflammatory sites. In this study, we investigated the structure/activity relationship of a series of flavonoids on the oxidative burst of human neutrophils in vitro, as a measure of its anti-inflammatory potential. Neutrophils were stimulated with phorbol-12-myristate-13-acetate, and fluorescence and chemiluminescence techniques were used to evaluate the generation of reactive oxygen species. All the tested flavonoids revealed the ability to modulate the neutrophil's oxidative burst. From the obtained results, the pivotal role of the catechol group in the B-ring was evidenced as well as the minor importance of the hydroxylations in the A-ring, which did not appear to be determinant for the activity, although clearly influencing the lipophilicity of the tested flavonoids. It is also clarified the importance of the methylation in the OH group at the B-ring catechol moiety. In conclusion, the obtained results uncover new possible strategies for the resolution of inflammatory processes, using flavonoids to modulate neutrophil's oxidative burst.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Medicinal Chemistry - Volume 67, September 2013, Pages 280–292
نویسندگان
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