Synthesis, characterization and antimicrobial activity of water soluble dendritic macromolecules

Several families of water soluble dendrimers were synthesized based on poly(propyleneoxide) amines (Jeffamines®) (P1). P1-core and branched units were constructed from both methylacrylate and ethylenediamine (P2–P9, and generations 0–3 with –NH2, –COOH functionalities). They were characterized by elemental analysis (EA), gel permeation chromatography (GPC), FT-IR, 1H, and 13C NMR. The antimicrobial activities of only water soluble compounds (P1, P3, P4, P6, P7 and P9) were evaluated using disk diffusion method in water as well as the minimal inhibitory concentration (MIC) dilution method against 9 bacteria. The obtained results from disk diffusion method are assessed in side-by-side comparison with those of Penicillin-g, Ampicillin, Cefotaxime, Vancomycin, Oflaxacin, and Tetracyclin, well-known antibacterial agents. The results from dilution procedure are compared with Gentamycin as antibacterial and Nystatin as antifungal. The antifungal activities are reported on five yeast cultures namely, Candida albicans, Kluyveromyces fragilis, Rhodotorula rubra, Debaryomyces hansenii, and Hanseniaspora guilliermondii, and the results are referenced with Nystatin, Ketaconazole, and Clotrimazole, commercial antifungal agents. In most cases, the compounds show broad-spectrum (Gram-positive and Gram-negative bacteria) activities that are comparatively higher or equipotent to the antibiotic and antifungal agents in the comparison tests.

Several water soluble dendrimers were synthesized based on poly(propyleneoxide) amines (P1) and branched units were constructed from both methylacrylate and ethylenediamine (P2–P9). They were characterized by elemental analysis, GPC, FT-IR, 1H and 13C NMR spectra. The antimicrobial activities were evaluated using disk diffusion method as well as the minimal inhibitory concentration (MIC) dilution method. Figure optionsDownload as PowerPoint slide