Tri- and diorganotin(IV) complexes of biologically important orotic acid: Synthesis, spectroscopic studies, in vitro anti-cancer, DNA fragmentation, enzyme assays and in vivo anti-inflammatory activities

Tri-and diorganotin(IV) orotates of general formula, RnSn(H2Or)m [n = 3/2, m = 1/2, R = Me, n-Bu, n-Oct and Ph; H2Or− = monoanion of orotic acid (H3Or)] (n-Bu2Sn(HOr) as an exception) have been synthesized. On the basis of various spectroscopic studies it is revealed that R3Sn(H2Or) and R2Sn(H2Or)2 exhibit distorted trigonal–bipyramidal and distorted octahedral geometry, respectively, and n-Bu2Sn(HOr) shows both five and six coordination geometry around tin. In vitro anti-cancer screening against MCF-7 (mammary), HEK-293 (kidney), PC-3 (prostate), HCT-15 (colon) and HepG-2 (liver) cancer cell lines suggest that the n-Oct2Sn(H2Or)2 is the most active complex among all of the studied complexes. DNA fragmentation and antioxidant enzyme assays suggest that cytotoxic effect of the complexes is selectively mediated through the induction of apoptosis. They also exhibit low toxicity and good anti-inflammatory activity (in vivo).

Organotin(IV) orotates have been synthesized and characterized. Di-n-octyltin(IV) orotate is the most active among the studied complexes and its cytotoxicity is selectively mediated through apoptosis. Complexes exhibit good anti-inflammatory activity.Figure optionsDownload as PowerPoint slideHighlights
► Tri- and di- organotin(IV) orotates have been synthesized and characterized.
► MTT assay suggests, n-Oct2Sn(H2Or)2 is the most active complex.
►  DNA ladder and enzyme activities indicate cell growth inhibition through apoptosis.
► In vivo anti-inflammatory activity and toxicity have been carried out.