کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1395336 | 1501204 | 2009 | 7 صفحه PDF | دانلود رایگان |
Star-burst dendrimers represent a superior carrier platform for targeted drug delivery. Partially acetylated generation 5 (G5) polyamidoamine (PAMAM) dendrimer was conjugated with the targeting moiety (biotin) and the imaging moiety (fluoresceinisothiocyanate, FITC), and the resulting dendrimer–biotin conjugate was characterized by 1H NMR, UV–vis spectrum. As revealed by flow cytometry and confocal microscopy, the bifunctional conjugate (dendrimer–biotin–FITC) exhibited much higher cellular uptake into HeLa cells than the conjugate without biotin. The uptake was energy-dependent, dose-dependent, and could be effectively blocked by dendrimer-conjugated biotin. Our results indicated that the biocompatible biotin–dendrimer conjugate might be a promising nano-platform for cancer therapy and cancer diagnosis.
Schematic representation of the reactions involved in the synthesis of multi-functional nanodevices based on PAMAM dendrimers for cancer cell targeting and imaging.Figure optionsDownload as PowerPoint slide
Journal: European Journal of Medicinal Chemistry - Volume 44, Issue 2, February 2009, Pages 862–868