کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1395958 | 1501169 | 2012 | 16 صفحه PDF | دانلود رایگان |
The Met receptor tyrosine kinase is a promising target in anticancer therapies for its role during tumor evolution and resistance to treatment. It is characterized by an unusual structural plasticity as its active site accepts different inhibitor binding modes. Such feature can be exploited to identify distinct agents targeting tumor dependence and/or resistance by oncogenic Met. Here we report the identification of bioactive agents, featuring a new 4-(imidazo[2,1-b]benzothiazol-2-yl)phenyl moiety, targeting cancer cells dependent on oncogenic Met. One of these compounds (7c; Triflorcas) impairs survival, anchorage-independent growth, and in vivo tumorigenesis, without showing side effects. Our medicinal chemistry strategy was based on an in-house Met-focused library of aminoacid-amide derivatives enriched through structure-based computer modeling, taking into account the Met multiple-binding-mode feature. Altogether, our findings show how a rational structure-based drug design approach coupled to cell-based drug evaluation strategies can be applied in medicinal chemistry to identify new agents targeting a given oncogenic-dependency setting.
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► The unusual plasticity of Met active site permits different inhibitor-binding-modes.
► Rational structure-based drug design was coupled to cell-based drug evaluation.
► New 4-(imidazo[2,1-b]benzothiazol-2-yl)phenyl agents target oncogenic Met signaling.
► Compound 7c impairs in vitro and in vivo Met-driven tumorigenesis.
► Findings exemplify how to identify agents targeting an oncogenic-dependency setting.
Journal: European Journal of Medicinal Chemistry - Volume 47, January 2012, Pages 239–254