7-Substituted pterins provide a new direction for ricin A chain inhibitors

Ricin is a potent toxin found in castor seeds. The A chain, RTA, enzymaticlly depurinates a specific adenosine in ribosomal RNA, inhibiting protein synthesis. Ricin is a known chemical weapons threat having no effective antidote. This makes the discovery of new inhibitors of great importance. We have previously used 6-substituted pterins, such as pteroic acid, as an inhibitor platform with moderate success. We now report the success of 7-carboxy pterin (7CP) as an RTA inhibitor; its binding has been monitored using both kinetic and temperature shift assays and by X-ray crystallography. We also discuss the synthesis of various derivatives of 7CP, and their binding affinity and inhibitory effects, as part of a program to make effective RTA inhibitors.

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► Several new pterin compounds were synthesized and analyzed.
► Many compounds showed good inhibition of ricin toxin A in an in vitro assay.
► Nearly all of these compounds were observed through X-ray crystallography to be bound within the active site of RTA.
► The success of these compounds indicates a greater affinity of 7-substituted pterins, compared to the regioisomeric 6-derivatives.