کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
154610 456846 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oxidative protein refolding on size exclusion chromatography: From batch single-column to multi-column counter-current continuous processing
ترجمه فارسی عنوان
کروماتوگرافی جدا سازی پروتئین اکسیداتیو: از یک سری تک ستون به چند ستون متقابل پردازش مداوم متقابل
کلمات کلیدی
پروتئین تشدید شده، اندازه کروماتوگرافی حذف، مدل سازی ریاضی، مجددا خاموش ستون
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی


• Mathematical model presented for its applicability for protein oxidative refolding/aggregation predictions in SMB-SEC.
• The model considers a wider loading concentration range of the model protein (lysozyme) on SEC.
• It was observed that at higher loading concentrations aggregation occurs when local protein concentration exceeds a critical concentration.
• The model was found to predict the SMB-SEC performance of solubilized protein recovery.
• No urea recovery at the product stream indicated that the refolding reaction will continue off-column to recover the native-protein product.

Recently protein refolding on size exclusion chromatography (SEC) operated in multi-column continuous simulated moving bed (SMB) configurations (hereinafter SMB-SEC) has been investigated for future industrial applications. This is due to several advantages offered by SMB configurations particularly when process parameters are thoroughly screened and optimized. A robust mathematical model is essential for high-throughput process screening and optimization. In this work, a previously investigated single-column mathematical model was modified to extend its applicability for protein oxidative refolding/aggregation predictions in SMB-SEC. The model considers a wide loading concentration range of the model protein (lysozyme) on SEC. The potential influences of high concentrations of chaotropic reagents on kinetic and thermodynamic model parameters have been discussed based on previous experimental results and their predicted local concentrations through the SMB-SEC columns and at the product stream. It was observed that aggregation occurs when local protein concentration exceeds a critical concentration. No urea recovery at the product stream indicated that the refolding reaction will continue off-column to recover the native-protein product. Therefore, it is suggested that the developed model is tested against experimental results for total soluble protein (early intermediates and native conformations) in the presence of ʟ-arginine additive and process performance indicators are defined based on this criterion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Science - Volume 138, 22 December 2015, Pages 375–384
نویسندگان
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