کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
155601 456905 2012 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Time evolution of amyloid fibril length distribution described by a population balance model
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Time evolution of amyloid fibril length distribution described by a population balance model
چکیده انگلیسی

The formation of toxic protein aggregates is thought to be the key event in several neurodegenerative human diseases. In the last years, the combination of experimental characterization and kinetics models based on mass action laws or molecular dynamics gained important insights into the mechanistic description of the aggregation process. In this work, we investigate the fibrillation of β-lactoglobulin, a common model protein for amyloid aggregation studies with relevant applications in the food industry. In addition to the determination of the fibrillation kinetics by Thioflavin-T, we measure the time evolution of the fibril length distribution during the aggregation process, in both stagnant and shaking conditions. A population balance equation model is used to simulate the experimental data. The model describes successfully the kinetics and the complete fibril length distribution in both conditions. We show that the description of the length distribution is fundamental in discriminating the correct mechanistic picture of the aggregation process. In particular, secondary nucleation due to length-dependent breakage is found to occur in both conditions, with larger extent under shaking conditions. However, it is found that, at least for the system under investigation, fragmentation by breakage alone cannot justify the absence of the lag phase when shaking is applied. This is also related to the effect of shaking on the primary nucleation rate and on the morphology of nuclei and fibrils formed in the early stages of aggregation. This effect is possibly to be attributed to the presence of hydrophobic air interfaces created through shaking.


► A population balance model is used to simulate beta-lactoglobulin fibril formation.
► The complete length distribution is considered together with monomer conversion.
► Shaking-induced fragmentation alone cannot justify the absence of the lag phase.
► Shaking affects also primary nucleation rate and morphology of nuclei.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Science - Volume 78, 20 August 2012, Pages 21–32
نویسندگان
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