کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
155635 | 456907 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Diffusive transfer between two intensely interacting cells with limited surface kinetics Diffusive transfer between two intensely interacting cells with limited surface kinetics](/preview/png/155635.png)
The diffusive transfer, or paracrine delivery, of chemical factors during the interaction of an emitting cell and a receiving cell is a ubiquitous cellular process that facilitates information exchange between the cells and/or to bystander cells. In the cellular immune response this exchange governs the magnitude and breadth of killing of cellular targets, inflammation or tolerance. Paracrine delivery is examined here by solving the steady-state diffusion equation for the concentration field surrounding two intensely interacting, equi-sized cells on which surface kinetics limits the rates of factor emission and absorption. These chemical factors may be cytokines, such as Interlukins and Interferons, but the results are presented in a generic form so as to be applicable to any chemical factor and/or cell-type interaction. In addition to providing overall transfer rates and transfer efficiencies, the results also indicate that when the receiving cell is naïve, with few factor receptors on its surface, there may be a significant accumulation of factor in the synaptic region between the cells with a consequent release of factor to the medium where it can signal bystander cells. This factor accumulation may play a critical role in activating a naïve receiving cell. As the receiving cell activates and becomes more absorbent, the factor accumulation diminishes, as does potential bystander signaling.
► Calculation of the concentration field near intensely interacting cells.
► Results show the effects of surface kinetics on intercellular concentration.
► Results show the effects of surface kinetics on transfer rates.
► Significant accumulation of transferred factor may occur near naïve cells.
► Perfect source/perfect sink interaction model is of limited utility.
Journal: Chemical Engineering Science - Volume 74, 28 May 2012, Pages 114–123