کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
157379 | 456970 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Utilizing simple biochemical measurements to predict lifetime output of biocatalysts in continuous isothermal processes Utilizing simple biochemical measurements to predict lifetime output of biocatalysts in continuous isothermal processes](/preview/png/157379.png)
The expected product yield of a biocatalyst during its useful lifetime is an important consideration when designing a continuous biocatalytic process. One important indicator of lifetime biocatalyst productivity is the dimensionless total turnover number (TTN). Here, a method is proposed for estimating the TTN of a given biocatalyst from readily measured biochemical quantities, namely the specific activity and the deactivation half-life, measured under identical conditions. We demonstrate that this method may be applied to any enzyme whose thermal deactivation follows first-order kinetics, regardless of the number of unfolding intermediates, and that the TTN method circumvents the potential problems associated with measuring specific catalyst output when a portion of the enzyme is already unfolded. The TTN estimation was applied to several representative biocatalysts to demonstrate its applicability in identifying the most cost-effective catalyst from a pool of engineered mutants with similar activity and thermal stability.
Journal: Chemical Engineering Science - Volume 65, Issue 6, 15 March 2010, Pages 2118–2124