Metabolic network flux analysis for engineering plant systems

Metabolic network flux analysis (NFA) tools have proven themselves to be powerful aids to metabolic engineering of microbes by providing quantitative insights into the flows of material and energy through cellular systems. The development and application of NFA tools to plant systems has advanced in recent years and are yielding significant insights and testable predictions. Plants present substantial opportunities for the practical application of NFA but they also pose serious challenges related to the complexity of plant metabolic networks and to deficiencies in our knowledge of their structure and regulation. By considering the tools available and selected examples, this article attempts to assess where and how NFA is most likely to have a real impact on plant biotechnology.

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► A powerful range of tools has been developed for metabolic network flux analysis.
► These tools yield insights that are used to aid microbial metabolic engineering.
► Plants present great opportunities and special challenges to applying these tools.
► Tool selection and knowledge of plant systems is key to practical success.