Rapid Preparation Process of Silver Nanoparticles by Bioreduction and Their Characterizations1

Bioreduction as a novel nanoparticle synthesizing technology attracts increasing attention. Dried cells of the bacterium Aeromonas sp. SH10 rapidly reduced [Ag(NH3)2]+ to Ag0 in the solution into which some amount of OH− was introduced. The surface plasmon resonance centered at 425 nm on the UV-vis spectra and five broad Bragg reflections on the XRD pattern showed that stable silver nanoparticles were formed during the bioreduction process. TEM and SEM observations suggested that the silver nanoparticles were uniform in size and well dispersed on the cells and in the solution. Therefore, silver nanoparticles could be prepared rapidly by this bioreduction technology.