Three-Dimensional Assembly of Multilayered Tissues

A rapid construction process is necessary for building up numerous cell modules into 3D tissues maintaining the tissue geometries and initial conditions of cells. We proposed new 3D assembly technique to fabricate a hollow tubular tissue structure using by water transfer printing. By utilizing this assembly technique, we discuss the relation between 3D transcriptional body of gel matrix and a developed figure of transfer tissue, and perform fabricating on a hollow tubular tissue. Appropriately simulation of the 3D environment in which tissues normally develop and function is crucial for the engineering in vitro models that can be used for the formation of complex tissues. These artificial hollow tubular tissues would be used for drug efficiency evaluation and operative training as in vitro simulators.