کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
175209 458905 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
چکیده انگلیسی

Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Data in Brief - Volume 3, June 2015, Pages 221–228
نویسندگان
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