کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
175883 458926 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ratiometric and colorimetric detection of hydrogen sulfide with high selectivity and sensitivity using a novel FRET-based fluorescence probe
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Ratiometric and colorimetric detection of hydrogen sulfide with high selectivity and sensitivity using a novel FRET-based fluorescence probe
چکیده انگلیسی


• A novel FRET-based ratiometric and colorimetric fluorescence probe for hydrogen sulfide was designed and synthesized.
• The probe is high sensitive to H2S with 15-fold fluorescence enhancement.
• The probe displays H2S selectivity with a detection limit of 0.4 μM.
• The probe can image H2S in living cells.

With recognition of the biological significance of H2S, we have designed and synthesized a novel ratiometric and colorimetric fluorescence probe, CR-DNP based on a H2S induced thiolysis reaction as well as the fluorescence resonance energy transfer process. It displayed some desirable properties, such as good photostability, high selectivity and excellent sensitivity. Also, a considerably fluorescence enhancement (15-fold) and distinct visible color change (colorless to bright orange) was observed. A good linear relationship between the emission intensity ratios and NaHS concentrations suggested that it can detect H2S quantitatively with a detection limit as low as 0.4 μM. Furthermore, due to its biocompatibility, the sensor could easily permeate through the cell membrane and trace the intracellular H2S levels change.

A novel ratiometric and colorimetric fluorescence probe CR-DNP for qualitative and quantitative detection of H2S was designed and synthesized based on a H2S induced thiolysis reaction as well as the fluorescence resonance energy transfer process.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 118, July 2015, Pages 88–94
نویسندگان
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