کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
175883 | 458926 | 2015 | 7 صفحه PDF | دانلود رایگان |

• A novel FRET-based ratiometric and colorimetric fluorescence probe for hydrogen sulfide was designed and synthesized.
• The probe is high sensitive to H2S with 15-fold fluorescence enhancement.
• The probe displays H2S selectivity with a detection limit of 0.4 μM.
• The probe can image H2S in living cells.
With recognition of the biological significance of H2S, we have designed and synthesized a novel ratiometric and colorimetric fluorescence probe, CR-DNP based on a H2S induced thiolysis reaction as well as the fluorescence resonance energy transfer process. It displayed some desirable properties, such as good photostability, high selectivity and excellent sensitivity. Also, a considerably fluorescence enhancement (15-fold) and distinct visible color change (colorless to bright orange) was observed. A good linear relationship between the emission intensity ratios and NaHS concentrations suggested that it can detect H2S quantitatively with a detection limit as low as 0.4 μM. Furthermore, due to its biocompatibility, the sensor could easily permeate through the cell membrane and trace the intracellular H2S levels change.
A novel ratiometric and colorimetric fluorescence probe CR-DNP for qualitative and quantitative detection of H2S was designed and synthesized based on a H2S induced thiolysis reaction as well as the fluorescence resonance energy transfer process.Figure optionsDownload as PowerPoint slide
Journal: Dyes and Pigments - Volume 118, July 2015, Pages 88–94