کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
176324 | 458940 | 2014 | 9 صفحه PDF | دانلود رایگان |
• A series of dimethine cyanine dyes were synthesized by a microwave-assisted method.
• The emission maxima of the dyes were in the region 471–569 nm in PBS.
• The dyes as fluorescent probes for cell imaging and flow cytometry were investigated.
• Three dyes could be applied to living cells imaging and flow cytometry.
A series of dimethine cyanine dyes, used as fluorescent probes, were synthesized under microwave irradiation, and identified by 1H NMR, IR, elemental analysis and HRMS. The investigation of their spectral properties in phosphate-buffer saline (PBS) showed that the absorption and emission maxima of the dyes were in the region 370–480 nm and 471–569 nm, respectively. The properties of the dyes as fluorescent probes for living cells imaging and flow cytometry were investigated. The results showed that dyes 1, 2, 8 or 9 could penetrate an intact cell membrane, stained the cell nuclear and exhibited bright fluorescence. Little background interference, low cell cytotoxicity and little photobleaching were showed during the imaging tests. The dyes 1, 8 and 9 could be applied in flow cytometry and dye 1/PI, dye 8/PI or dye 9/PI couple could be proposed as double staining agents to measure sperm cell viability. Thus the dyes represented the cell-permeant fluorescent probes.
A series of dimethine cyanine dyes were synthesized. The potential applications of the dyes as fluorescent probes were explored in living cell imaging and flow cytometry.Figure optionsDownload as PowerPoint slide
Journal: Dyes and Pigments - Volume 100, January 2014, Pages 232–240