کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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176619 | 458950 | 2013 | 6 صفحه PDF | دانلود رایگان |
A class of hydrophilic, photostable, biocompatiable, and highly fluorescent streptavidin-functionalized silica nanoparticles were developed, in which Rhodamine B isothiocyanate molecules were covalently embedded by using Stöber method. The silica nanoparticles were ∼60 nm in size and had average fluorescence intensity 4000 times higher than dye molecules. To reduce the severe aggregation, silica nanoparticles were modified with amino groups on the surface by adding (3-aminopropyl)triethoxysilane together with methylphosphonate groups using 3-trihydroxysilylpropylmethylphosphonate, followed by functionalization with streptavidin. The resulting streptavidin-functionalized silica nanoparticles were used for the detection of by reverse-phase protein microarrays based on classical linkage system constituted by biotin-streptavidin conjugation. The calibration curve was linear over the range from 40 amol to 640 amol and the limit of detection was 10 amol, which was 8 times lower than that of streptavidin-labeled cyanine fluorescent dyes. This promising technology may be potential applied for multiplexed signaling and bioassays.
► Highly fluorescent Rhodamine B doped core–shell silica nanoparticles were developed.
► The nanoparticles were used for the detection of human IgE by protein microarrays.
► This probe has higher sensitivity than a traditional fluorescent cyanine dye.
Journal: Dyes and Pigments - Volume 98, Issue 1, July 2013, Pages 119–124