A selective fluorescent chemosensor with 1, 2, 4-triazole as subunit for Cu (II) and its application in imaging Cu (II) in living cells

A chemosensor based on rhodamine B with 1, 2, 4-triazole as subunit was synthesized and characterized. It exhibits high selectivity and sensitivity for Cu2+ in ethanol/water (6:4, v:v) of pH 7.0 HEPES buffer solution and undergoes ring opening mechanism, and a 2:1 metal–ligand complex is formed. The chemosensor displays a linear response to Cu2+ in the range between 1.0 × 10−7 M and 1.0 × 10−6 M with a detection limit of 4.5 × 10−8 M. Its capability of biological application was also evaluated and the results show that this chemosensor could be successfully employed as a Cu2+-selective chemosensor in the fluorescence imaging of living cells.

A novel fluorescent chemosensor L bearing a 1, 2, 4-triazole ring was designed to detect Cu(II) in ethanol–water solution (6:4, v/v, 50 mM HEPES, pH 7.0) with remarkable fluorescence enhancement and clear color change, and its high cell permeability grants its application to fluorescent imaging in living cells.Figure optionsDownload as PowerPoint slideHighlights
► An “off–on” Cu2+-selective probe was characterized.
► Triazole was introduced into the probe as subunit.
► It was successfully used for the detection of Cu2+ in the living cells.