Synthesis of novel cyanine dyes containing carbamoylethyl component – Noncovalent labels for nucleic acids detection

Fourteen novel mono-, di- and tricationic monomethine cyanine dyes belonging to the Thiazole Orange family have been prepared via an improved synthetic procedure. The dyes, useful for nucleic acid detection, bear carbamoylethyl substituents. All derivatives absorb in the region 428–515 nm and have a molar absorptivity of 58 000–91 000 l. mol−1. cm−1. The reaction products were characterized by 1H-NMR spectroscopy and elemental analysis.