Investigation of electrochemical behavior of anisomycin on gold electrode followed by HPLC–MS/MS analysis

• For the first time anisomycin was investigated by voltammetry techniques.
• Sensitive electrochemical method for quantification of anisomycin was developed.
• It was applied for the determination of anisomycin and in biological samples.
• Long term potential cycling was followed by HPLC–MS/MS analysis.
• Anisomycin hydrolysis product was defined as deacetylanisomycin.

Anisomycin is an immunosuppressant in low doses (< 0.1 μM) with possible application in treatment of some autoimmune diseases and in inhibiting transplantation rejection. Anisomycin suppresses malignant tumor cell growth and affects memory. For the first time it was the subject of the electrochemical investigations by cyclic voltammetry and square wave voltammetry on gold electrode in 0.05 M NaHCO3 using its electrochemical activity. The cyclic voltammetry experiments at different sweep rates show that electrochemical process is irreversible and diffusion controlled. Based on square wave voltammetry measurements, the calculated values of LOD and LOQ were 1 and 4 nM (in the absence of biological fluid), as well as 2 and 6 nM (in the presence of spiked urine) indicating the high sensitivity of the proposed electroanalytical method. High performance liquid chromatography–tandem mass spectrometry was a reference method for quantification of anisomycin and served for structural identification of its hydrolysis product (deacetylanisomycin).

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