Cyclic voltammetric and nanogravimetric studies of NADP+ redox transformations on a yeast-modified platinum electrode

• Preparation of a yeast-modified electrode
• Application of a yeast-modified electrode for the quantitative determination of NADP+
• Reversible voltammetry of NADP+
• Surface mass changes during the cyclic voltammetry of NADP+

The dominant voltammetric response of a yeast suspension in neutral or slightly alkaline media can be assigned to the redox transformations of nicotinamide adenine dinucleotide (NAD+/NADH) and nicotinamide adenine dinucleotide phosphate (NADP+/NADPH). By immobilization of yeast on platinum, a stable electrode can be prepared which shows an electrocatalytic activity towards the reduction of NADP+ and the reoxidation of the product formed. Reversible cyclic voltammetric responses were obtained. The peak currents depend practically linearly on the NADP-Na2 concentration and on the square root of the scan rate. The surface mass changes accompanying the redox transformations were monitored by an electrochemical quartz crystal nanobalance.

Figure optionsDownload as PowerPoint slide