کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
179050 | 459334 | 2014 | 4 صفحه PDF | دانلود رایگان |
• Electrochemical method for transglutaminase activity
• Cyclic voltammetry for non-redox enzyme assay
• Gold patterned with molecular nanopores for the enzyme-controlled diffusion of Fe(CN)63 −/4 −
• Mixed monolayer of aminated β-cyclodextrin/1-octanethiol as substrate for transglutaminase
A novel electrochemical method for measuring transglutaminase activity was reported. This approach was based on the patterning of gold electrodes with a mixed self-assembled monolayer of perthiolated β-cyclodextrin and 1-octanethiol. The proper functionalization of β-cyclodextrin with primary amino groups allowed it to act as amino-donor substrates for transglutaminase and molecular nanopores for the enzyme-controlled diffusion of Fe(CN)63 −/4 − to the electrode surface. Voltamperometric measurements allowed detection of transglutaminase in the range of 1.9–37 mU/mL with a sensitivity of 1.42 nA μL U− 1 s− 1.
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Journal: Electrochemistry Communications - Volume 40, March 2014, Pages 13–16