Electrochemically induced deposition of poly(benzoxazine) precursors as immobilization matrix for enzymes

• Non-manual electrochemically induced polymerization of benzoxazine derivatives
• Controlled deposition of poly(benzoxazine) by optimized electrochemical pulse sequences
• Entrapment of glucose oxidase in electropolymerized poly(benzoxazine) films

Water-soluble benzoxazine oligomers were synthesized by reacting bisphenol A, tetraethylenepentamine and formaldehyde. The pre-formed benzoxazine oligomers can be further electropolymerized from aqueous suspensions under formation of poly(benzoxazine)-based films on electrode surfaces. Integration of glucose oxidase in a poly(benzoxazine) film let to highly reproducible and stable biosensors. Poly(benzoxazines) are proposed as a new family of stable polymers for the design of enzyme electrodes.