Label free MUC1 aptasensors based on electrodeposition of gold nanoparticles on screen printed electrodes

• Two electrochemical aptasensors for MUC1 tumor marker detection are presented.
• The thiolated aptamers were immobilized on AuNP on graphite and gold SPE.
• The MUC1 detection limits are 3.6 ng mL− 1 by EIS and 0.95 ng mL− 1 by DPV.

Two simple electrochemical aptasensors were developed for the detection of MUC1 tumor marker based on its specific recognition by thiolated aptamers immobilized on gold nanoparticle (Au NP)-modified graphite and gold screen printed electrodes (SPEs). The quantitative detection of MUC1 protein was achieved by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). Calibration curves were obtained with both techniques under optimized conditions. The estimated detection limits of the MUC1 protein are 3.6 ng mL− 1 at Au NP-modified graphite SPE by EIS and 0.95 ng mL− 1 at Au NP-modified gold SPE by DPV methods. The results demonstrated that the electrochemical method using aptamer probe immobilized on Au NPs is convenient and allows the quantitative detection of MUC1 protein.

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