کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1802522 1024598 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of magnetic poly(styrene–glycidyl methacrylate) microspheres for immunomagnetic separation of bone marrow cells
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم فیزیک ماده چگال
پیش نمایش صفحه اول مقاله
Application of magnetic poly(styrene–glycidyl methacrylate) microspheres for immunomagnetic separation of bone marrow cells
چکیده انگلیسی

Surface-functionalized magnetic poly(styrene–glycidyl methacrylate) (PS–GMA) microspheres were prepared and coupled with Sca-1 antibody for cell selection from murine bone marrow mononuclear cells (MNCs). Biotinylated Sca-1 antibody could be directly coupled to avidin-bound magnetic microspheres. Alternatively, oxidized goat anti-mouse antibody was covalently bound onto the amino group-containing magnetic microspheres in a site-directed manner, and the resultant conjugate was coupled with non-modified Sca-1 antibody. Using the indirect antibody-bound magnetic microspheres, the purity of isolated Sca-1+ cells increased with bead-to-cell ratio. Using a bead-to-cell ratio of 10 beads/cell, a purity of 85% Sca-1+ cells corresponding to a 17-fold enrichment was achieved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetism and Magnetic Materials - Volume 321, Issue 10, May 2009, Pages 1635–1638
نویسندگان
, , ,