کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
180393 | 459379 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Gold sputtered electrode surfaces enhance direct electron transfer reactions of human cytochrome P450s Gold sputtered electrode surfaces enhance direct electron transfer reactions of human cytochrome P450s](/preview/png/180393.png)
We immobilized human cytochrome P450 (CYP), a membrane-bound enzyme, onto both smooth and nanostructured surfaces of gold electrodes via a naphthalene thiolate monolayer film. Rapid electron transfer of CYP with an electrode as a redox partner took place when the enzyme was immobilized onto an electrode surface with nanostructures. This structure was easily prepared by conventional sputtering techniques. A well-defined pair of peaks was observed at − 0.175 V (vs. SHE) with the largest heterogeneous electron transfer rate constant of 340 s− 1 for human CYP. The positive redox potential shift of 45 mV upon drug (testosterone) binding was clearly detected, which corresponded to a change in the spin states of heme iron in CYP. The present study showed that gold sputtered surfaces are very useful for direct electron transfer reactions of human CYP isoforms.
Journal: Electrochemistry Communications - Volume 12, Issue 5, May 2010, Pages 680–683