کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
180723 | 459387 | 2010 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Microfluidic chip-based aptasensor for amplified electrochemical detection of human thrombin Microfluidic chip-based aptasensor for amplified electrochemical detection of human thrombin](/preview/png/180723.png)
In this paper, a microchip-based sandwich-type aptasensor is developed for the detection of human thrombin. The SH-aptamer/thrombin/alkaline phosphatase-functionalized aptamer (ALP-aptamer) system was constructed in the microfluidic channels. And the substrate solution containing 4-aminophenyl phosphate (p-APP) was introduced to the microchannels for the end-column electrochemical detection. The on-chip aptasensor has a broad linear response range of 1–100 pM with a detection limit of 1 pM, which shows high sensitivity and specificity. The system was then applied to detect thrombin in human serum sample. Therefore, the on-chip aptasensor has a great promise for detecting and screening ultratrace levels of biomarkers in the complex matrices.
Journal: Electrochemistry Communications - Volume 12, Issue 2, February 2010, Pages 258–261