کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
181028 459394 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrasensitive detection based on an aptamer beacon electron transfer chain
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Ultrasensitive detection based on an aptamer beacon electron transfer chain
چکیده انگلیسی

The construction of an ultrasensitive, reagentless, target label free electrochemical aptamer sensor (aptasensor) for thrombin detection is described. The aptasensor is based on a chronoamperometric beacon system for biomolecular recognition. The ferrocene-labeled aptamer adopts a 3-D conformational change when interacts with thrombin. Thus the ferrocene label is approached to the microperoxise-11 (MP-11) attached on the electrode surface. The thrombin–aptamer interaction is detected via a microperoxidase mediated electron transfer between the ferrocene and the surface. This system was demonstrated with surface plasmon resonance, impedance spectroscopy and chronoamperometry measurements, obtaining higher sensitivity (30 fM) with impedance spectroscopy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 10, Issue 10, October 2008, Pages 1533–1536
نویسندگان
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