کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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181195 | 459397 | 2009 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Direct evidence of electron flow via the heme c group for the direct electron transfer reaction of fructose dehydrogenase using a silver nanoparticle-modified electrode Direct evidence of electron flow via the heme c group for the direct electron transfer reaction of fructose dehydrogenase using a silver nanoparticle-modified electrode](/preview/png/181195.png)
The direct electron transfer reaction of fructose dehydrogenase (FDH) from Gluconobacter sp. on alkanethiol-modified silver nanoparticles (AgNPs) was examined using cyclic voltammetry and surface-enhanced resonance Raman scattering (SERRS). Using cyclic voltammetry, catalytic oxidation currents (based on the direct electron transfer reaction of FDH) were observed from a potential of approximately −100 mV (vs. Ag/AgCl, 3 M NaCl) in the presence of d-fructose, without a mediator. A comparison of the SERRS spectra and the resonance Raman spectra of FDH in solution indicated that the heme c site retained its six-coordinated low-spin heme after immobilization. Moreover, SERRS also demonstrated that the heme c of the adsorbed FDH was the electron transfer site within the enzyme.
Journal: Electrochemistry Communications - Volume 11, Issue 8, August 2009, Pages 1623–1626