کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
181196 459397 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel protein analytical method based on hybrid-aptamer and DNA-arrayed electrodes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A novel protein analytical method based on hybrid-aptamer and DNA-arrayed electrodes
چکیده انگلیسی

A novel protein assay method based on a DNA array was developed, in which human immunoglobulin E (hIgE) and its DNA aptamer were used as an analytical model. The target protein hIgE was captured by the aptamer in homogeneous solution and then the resulting hIgE-aptamer complex was hybridized onto probes self-assembled on the DNA array. Measured by electrochemical impedance spectroscopy (EIS), the charge transfer resistance (Rct) of electrodes before and after hybridization was compared. To test the selectivity of the method, four different probes with one to three mismatched bases were immobilized on respective electrodes. The results showed that the complex could be hybridized and detected out on the electrodes modified with the fully complementary sequences. In addition, the DNA array could be employed to analyze multiple samples selectively with the matched aptamer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 11, Issue 8, August 2009, Pages 1627–1630
نویسندگان
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