کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
181239 | 459398 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: An amperometric immunosensor with a thiolated Protein G scaffold An amperometric immunosensor with a thiolated Protein G scaffold](/preview/png/181239.png)
The orientation of the analyte-specific capture antibody on the surface of electrochemical immunosensors plays an important role on their overall performance. We have employed a self-assembled layer of Protein G that was thiolated with succinimidyl-6-[3′-(2-pyridyldithio)-propionamido] hexanoate for the orientation-controlled immobilisation of a capture antibody in a flow-type amperometric immunosensor based on a two-site sandwich immunoassay. After establishing the formation of the thiolated Protein G layer on 1-mm screen printed electrodes, amperometric immunosensors for the detection of the hormone, human chorionic gonadotrophin (hCG), were successfully constructed upon this scaffold. These sensors were characterised by a limit of detection (based on three times the standard deviation of the blank signal) of 175 IU l−1 and a linear response up to approximately 5000 IU l−1 of hCG.
Journal: Electrochemistry Communications - Volume 10, Issue 7, July 2008, Pages 1020–1023