کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
181453 459403 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
TNT determination based on its degradation by immobilized HRP with electrochemical sensor
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
TNT determination based on its degradation by immobilized HRP with electrochemical sensor
چکیده انگلیسی

Based on the mechanism of 2,4,6-Trinitrotoluene (TNT) degradation, an amperometric hydrogen peroxide biosensor was constructed for the determination of trace amounts of TNT by immobilization of MWCNTs, HRP and Nafion onto the surface of glassy carbon electrode (GCE). The Nafion/MWCNTs/HRP biosensor was capable of degrading TNT with the consumption of H2O2 and HRP in 0.2 mol/L PBS (pH 7.0). Trace TNT was quantitative analyzed by the current decrease of H2O2 at the reductive potential of −0.35 V using cyclic voltammetry (CV). Effect of the ratio of MWCNTs/HRP, initial concentration of H2O2 and electrolyte’s pH were also optimized by CV. Under the optimal conditions, the current decrease of H2O2 that was consumed by TNT degradation was proportional to TNT ranging from 8.8 × 10−9 mol/L to 2.64 × 10−7 mol/L with a detection limit of 3.0 × 10−9 mol/L (S/N = 3). It developed a new way for simple, rapid and sensitive measurement of trace TNT.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 10, Issue 8, August 2008, Pages 1176–1179
نویسندگان
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