Anodic electrochemiluminescent behavior of lucigenin on MWNT/GCE

The electrochemiluminescent (ECL) behavior of lucigenin on a multiwall carbon nanotubes modified glassy carbon electrode (MWNT/GCE) during anodic scanning was studied. A strong and stable anodic ECL signal was found on MWNT modified electrode, which results from the oxidation reaction between lucigenin and the oxidation production of OH-. The effects of electrode materials, pH and scan rate on the ECL intensity were studied, and the possible ECL mechanism was also proposed. Under the optimized conditions, the ECL intensity was found to be linear with concentration of lucigenin in the range of 5.0 × 10−7–5.0 × 10−6 mol/L with a detection limit of 2.0 × 10−7 mol/L. Superoxide dimutase (SOD) was found to be able to inhibit this ECL system, based on which a sensitive ECL methods for detection of SOD had been established.